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Even light microscopy is often inaccessible because of the requisite infrastructure and technical expertise [ 7131617 ]. Without proper diagnostic tools, health care workers may misidentify and inappropriately triage cases of severe malaria [ 7 ]. An ideal diagnostic test would enable health care workers, especially nurses, midwives, and community health workers, to accurately and quickly identify patients either with or at risk for complications of severe malaria and initiate expedited management practices.
Rapid diagnostic tests RDTs are low—cost, simple tools for the diagnosis of malaria and are particularly valuable in settings where microscopy is not readily available [ 18 - 21 ]. Newer generation RDTs that incorporate multiple antigens on a single membrane have been developed to differentiate between various Plasmodium species infections. Additionally, these RDTs have shown some ability to provide a quantitative estimate of parasite density due to the differing sensitivities of the respective antigens [ 22 - 24 ].
Because parasite density can be a correlate of disease severity, the use of multiple antigens on a single membrane has the potential to aid in detection of severe malaria.
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No study, however, has examined the association of RDT band positivity with clinical parameters. Thus, we reviewed data from a peripheral health center in rural Uganda to assess for a correlation between band RDT positivity and markers of disease severity. Our overarching goal was to explore the potential use of a multiple—antigen RDT as a low—cost, scalable marker of patients at risk of severe malaria—related anemia.
The health center functions as a referral center for the Bugoye sub—county, and serves a rural population of approximately 50 residents. The center houses a small laboratory with staff trained to perform light microscopy and RDTs, as well other basic tests, such as hemoglobin estimation. Study procedures We reviewed data on all individuals with fever who underwent an RDT for suspected malaria infection. We abstracted data on patient age, sex, village of residence, patient disposition, laboratory results, and medication prescriptions from clinic registers.
The presence of a single HRP—2 line denotes infection with P. RDTs Lot for the study were obtained from Kampala Pharmaceutical Industries Kampala, Ugandaand stored in their original packaging at room temperature. In cases in which the control line did not appear, the result was considered invalid and the test was repeated.
When available, we reviewed matched thick and thin smears for specimens with three—band positive tests. Asexual parasitemia of any level was reported as a positive smear. Vatican-approved carbon-dating tests on fibers taken from the cloth in indicated that the shroud dated to. The Civil War, Part 2: Although photography was still in its infancy.
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